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中国精品科技期刊2020
谷雅文,于鲲鹏,任玉文,等. 一株高浓度氨氮耐受的除氨氮菌筛选、鉴定及发酵条件优化[J]. 食品工业科技,2021,42(7):110−118. doi: 10.13386/j.issn1002-0306.2020060116.
引用本文: 谷雅文,于鲲鹏,任玉文,等. 一株高浓度氨氮耐受的除氨氮菌筛选、鉴定及发酵条件优化[J]. 食品工业科技,2021,42(7):110−118. doi: 10.13386/j.issn1002-0306.2020060116.
GU Yawen, YU Kunpeng, REN Yuwen, et al. Isolation and Identification of an Ammonia Nitrogen Removal Bacteria Strain with High Tolerance to Ammonia Nitrogen and Optimization of Its Removal Condition[J]. Science and Technology of Food Industry, 2021, 42(7): 110−118. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060116.
Citation: GU Yawen, YU Kunpeng, REN Yuwen, et al. Isolation and Identification of an Ammonia Nitrogen Removal Bacteria Strain with High Tolerance to Ammonia Nitrogen and Optimization of Its Removal Condition[J]. Science and Technology of Food Industry, 2021, 42(7): 110−118. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060116.

一株高浓度氨氮耐受的除氨氮菌筛选、鉴定及发酵条件优化

Isolation and Identification of an Ammonia Nitrogen Removal Bacteria Strain with High Tolerance to Ammonia Nitrogen and Optimization of Its Removal Condition

  • 摘要: 利用摇瓶富集培养以及平板驯化筛选的方法,从受发酵工业污水污染严重的土壤中分离筛选得到高氨氮耐受的除氨氮菌株N-2。根据形态特征及基于16S rDNA序列的系统发育分析进行菌株鉴定;以高浓度氨氮模拟废水试验验证菌株最高氨氮耐受浓度;并以氨氮去除率为评价指标,通过单因素及正交试验优化确定菌株最佳氨氮去除条件,为其工业化应用奠定理论基础。结果表明:筛选得到一株综合性能优良的N-2菌株,经鉴定为球形赖氨酸芽孢杆菌(Lysinibacillus sphaericus);通过氮素形态测定表明该菌经细菌同化作用可在10 h内对氨氮实现快速去除,无硝酸盐氮与亚硝酸盐氮积累,且在氨氮初始浓度为6000 mg/L的模拟氨氮废水中仍能进行正常生长繁殖;优化确定最佳氨氮去除条件为C/N为10、接种量5%、pH8.0、温度31 ℃、转速180 r/min和装液量62.5 mL/250 mL;且在最优条件下对50 mg/L氨氮10 h去除率可达97.7%,对100 mg/L氨氮10 h去除率为91.0%,对500 mg/L氨氮10 h去除率为71.7%。由此可见,菌株N-2在高氨氮浓度发酵工业废水的氨氮去除具有广阔发展前景。

     

    Abstract: An ammonia nitrogen removal bacteria strain N-2 with high tolerance to ammonia nitrogen was isolated and screened from soil heavily polluted by fermentation industrial wastewater by means of shake flask enrichment culture and plate domestication screening. Then the strain N-2 was identified through its morphological characteristics and phylogenetic analysis based on its 16S rDNA sequences. The maximum ammonia nitrogen tolerance concentration of the strain was verified by the simulated wastewater with high concentration ammonia nitrogen test. The optimum removal condition of N-2 was optimized by single factor and orthogonal test with the target of ammonia nitrogen removal rate to establish the theoretical foundation for its industrial application. The results showed that the N-2 strain with excellent comprehensive performance was identified as Lysinibacillus sphaericus. The N-2 strain could rapidly remove ammonia nitrogen in the simulated ammonia nitrogen wastewater within 10 h by bacterial assimilation without any accumulation of nitrate and nitrite, and could also grow normally with initial concentration of ammonia nitrogen up to 6000 mg/L. The best ammonia nitrogen removal conditions were C/N 10, inoculation volume 5%, pH8.0, temperature 31 ℃, speed 180 r/min and liquid volume 62.5 mL/250 mL. Under the optimal conditions, the removal rate of ammonia nitrogen was up to 97.7% for the initial concentration was 50 mg/L, 91.0% for 100 mg/L and 71.7% for 500 mg/L within 10 h. To sum up, the strain N-2 can not only quickly and efficiently remove ammonia nitrogen in water, but also has high tolerance to ammonia nitrogen. It can be used as a potential strain resource for removing ammonia nitrogen in industrial wastewater with high ammonia nitrogen concentration.

     

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