Abstract: Objective:An ultra-high performance liquid chromatography-tandem mass spectrometry method for detection of furoxime,spironolactone and bumetanilan in chicken and swine,which were banned in animal-derived foods,was established. Methods:The samples were purified with C₁₈ powder after the extraction of ethyl acetate. The chromatographic column was Hypersil GOLD C₁₈(1.9 μm,2.1 mm×50 mm).Mobile phase was acetonitrile and 0.1% formic acid water. The flow rate was 0.4 mL/min,and the column temperature was 40 ℃. The electrospray was operated in both positive and negative mode at the same time and the samples were detected by multiple reaction monitoring(MRM).Results:The liner relations of the three diuretics were good within the concentration range of 1.0~500.0 μg/L(r>0.996). The average standard recovery rate was 67.3%~111.6%. Limits of detection of the method were 0.5 μg/kg and limits of quantification were 1.0 μg/kg. Conclusion:This method is simple and accurate,and can be used as a rapid method for detection of diuretics in chicken and swine.