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中国精品科技期刊2020
杨芳, 杨莉, 张振宇, 肖岚, 秦涛, 贾洪锋. 基于气相-离子迁移谱对竹燕窝菌汤风味成分的分析[J]. 食品工业科技, 2020, 41(23): 8-14,24. DOI: 10.13386/j.issn1002-0306.2020050191
引用本文: 杨芳, 杨莉, 张振宇, 肖岚, 秦涛, 贾洪锋. 基于气相-离子迁移谱对竹燕窝菌汤风味成分的分析[J]. 食品工业科技, 2020, 41(23): 8-14,24. DOI: 10.13386/j.issn1002-0306.2020050191
YANG Fang, YANG Li, ZHANG Zhen-yu, XIAO Lan, QIN Tao, JIA Hong-feng. Analysis of Volatile Flavor Constituents in Bamboo Bird’s Nest Soups with Headspace-Gas Chromatography-Ion Mobility Spectrometry[J]. Science and Technology of Food Industry, 2020, 41(23): 8-14,24. DOI: 10.13386/j.issn1002-0306.2020050191
Citation: YANG Fang, YANG Li, ZHANG Zhen-yu, XIAO Lan, QIN Tao, JIA Hong-feng. Analysis of Volatile Flavor Constituents in Bamboo Bird’s Nest Soups with Headspace-Gas Chromatography-Ion Mobility Spectrometry[J]. Science and Technology of Food Industry, 2020, 41(23): 8-14,24. DOI: 10.13386/j.issn1002-0306.2020050191

基于气相-离子迁移谱对竹燕窝菌汤风味成分的分析

Analysis of Volatile Flavor Constituents in Bamboo Bird’s Nest Soups with Headspace-Gas Chromatography-Ion Mobility Spectrometry

  • 摘要: 为探究竹燕窝菌汤风味物质的含量及变化规律,以筛选熬制竹燕窝菌汤的最佳熬制方式,以微波、电压力锅、电磁炉3种加热方式熬制的竹燕窝菌汤(分别标记为WB、DYL和DCL)作为试验对象,采用氨基酸自动分析仪和气相-离子迁移谱(GC-IMS)联用仪,根据相对气味活度值(ROAV)和主成分分析法(PCA)对竹燕窝菌汤的风味进行分析。结果表明,固形物溶出率、游离氨基酸总量最高的均为DYL,游离氨基酸中谷氨酸含量最高。GC-IMS二维图谱、指纹图谱均可得出三种样品挥发性有机物(VOCs)的明显差异,指纹图谱中B框主要存在于WB中,包括:1-辛醇、3-甲基丁酸二聚体等;D框主要存在于DCL中,包括:2-辛酮、壬醛等;F框主要存在于DYL中,包括:2-甲基丙醛、2-丙醇等。在2-甲基丙醛、2-丙醇含量方面,DYL与其余两种样品间存在显著性差异(P<0.05);已定性的VOCs中,以醛类和醇类化合物为主。ROAV法确定出三种样品的关键风味化合物种类相似,但贡献度差异较大。PCA分析显示,PC_161%与PC_230%之和为91%,PC_161%可将三种样品进行很好的区分。电压力锅熬制为竹燕窝菌汤的最佳熬制方式。基于GC-IMS检测技术,测定竹燕窝菌汤的VOCs,以对微波、电压力锅、电磁炉3种熬制工艺进行区分是可行的。

     

    Abstract: The content and regularity of volatile components in bamboo bird’s nest soups were explored to screen the best way to prepare bamboo bird’s nest soups. The bamboo bird’s nest soups made by microwave(WB),electric pressure cooker(DYL)and induction cooker(DCL). These samples were analyzed by amino acid automatic analyzer and gas chromatography-ion transfer spectrometry(GC-IMS)according to the relative odor activity value(ROAV)method and principal component analysis(PCA).The results showed that,DYL had the highest dissolution rate of solids and the highest total amount of free amino acids,while,the content of glutamic acid in free amino acid was the highest. The obvious differences of volatile organic compounds(VOCs)in three samples could be obtained by GC-IMS two-dimensional spectrum and fingerprint. In the fingerprint,the VOCs including 1-octanol,3-methyl butyric acid dimer and so on,as marked in frame B,mainly existed in WB. The VOCs including 2-octanone,nonanal,and so on,as marked in frame D,mainly existed in DCL. The VOCs including 2-methyl propionaldehyde,2-propanol and so on,as marked in frame F,mainly existed in DYL. There were significant differences in the contents of 2-methyl propionaldehyde and 2-propanol between DYL and the other samples(P<0.05). Aldehydes and alcohols were the main compounds in the identified VOCs. The result via ROAV method showed that the key flavor compounds of the three samples were similar,but the contribution was quite different. PCA analysis showed that the sum of PC1 61% and PC2 31% could distinguish the three samples well. Electric pressure cooker is the best tool for the preparation of bamboo bird’s nest soups. Based on the GC-IMS detection technology,it is feasible to distinguish the three cooking methods of bamboo bird’s nest soups,including WB,DYL and DCL,by detecting the VOCs.

     

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