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中国精品科技期刊2020
丁博群,刘珊娜. 荧光定量PCR技术在食品快速检测中的应用[J]. 食品工业科技,2021,42(7):366−373. doi: 10.13386/j.issn1002-0306.2020050185.
引用本文: 丁博群,刘珊娜. 荧光定量PCR技术在食品快速检测中的应用[J]. 食品工业科技,2021,42(7):366−373. doi: 10.13386/j.issn1002-0306.2020050185.
DING Boqun, LIU Shanna. Application of Real-time Quantitative PCR Technology in Food Rapid Detection[J]. Science and Technology of Food Industry, 2021, 42(7): 366−373. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020050185.
Citation: DING Boqun, LIU Shanna. Application of Real-time Quantitative PCR Technology in Food Rapid Detection[J]. Science and Technology of Food Industry, 2021, 42(7): 366−373. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020050185.

荧光定量PCR技术在食品快速检测中的应用

Application of Real-time Quantitative PCR Technology in Food Rapid Detection

  • 摘要: 荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)是一种将聚合酶链式反应(polymerase chain reaction,PCR)扩增与实时检测相结合的技术,也是近年来食品快速检测技术的研究热点之一。与传统PCR技术相比,它具有耗时短、操作简单、灵敏度高、特异性强等优势。本文简要概述了荧光定量PCR技术的基本原理、发展历程、分类及特点,综述了荧光定量PCR技术在食源性致病菌、掺杂掺假、转基因食品、过敏原、食源性寄生虫、可食用昆虫等食品检测中的应用,并对荧光定量PCR技术及其在食品快速检测中应用的前景进行了展望。

     

    Abstract: Real-time quantitative polymerase chain reaction (qPCR) is a technology that combines polymerase chain reaction (PCR) amplification with real-time detection, and also one of the research hotspots of food rapid detection technology in recent years. It has the advantages of short time-consuming, simple operation, high sensitivity, and strong specificity, compared with traditional PCR technology. This article briefly outlines the basic principles, development process, classification, and characteristics of real-time qPCR technology. The application in the detection of food-borne pathogens, adulteration, genetically modified foods, allergens, food-borne parasites, and edible insects is reviewed. The prospects of real-time qPCR technology and its application in food rapid detection are predicted.

     

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