Abstract: In order to analyze and evaluate the nutritional value of Akebia trifoliata fruit,Flame Atomic Absorption Spectrometry,Liquid Chromatography,Fluorescence Spectrophotometry and other experimental methods was used to detect and analyze the nutritional components in the peel,pulp and seed of Akebia trifoliata. Based on the detection and analysis,it could be seen that Akebia trifoliata fruit had high nutritional value. Among them:The peel of Akebia trifoliata contains mineral nutrients of Mn and K,the contents of Mn and K were 0.645 and 342 mg/100 g respectively. The total amino acid content in the peel of Akebia trifoliata was 0.313 mg/100 g,containing 15 kinds of amino acids,including 7 kinds of essential amino acids. The proportion of essential amino acids reached the ideal protein conditions proposed by WHO/FAO. The content of essential amino acids was less than that of pulp,and the nutritional value was lower than that of pulp. It contained a small amount of vitamin E,pectin,protein and fat. The seed of Akebia trifoliate contained many mineral nutrients,including K,P,Ca and Fe,with the contents of 586,180,191 and 2.85 mg/100 g,respectively. The seed of Akebia trifoliata was rich in many mineral nutrients,including Mn,Cu and Mg,with the contents of 4.63,1.3 and 157 mg/100 g,respectively. The fatty acid content was 2.662 g/100 g,and the proportion of unsaturated acid was 76%. The pulp of Akebia trifoliate contains Cu mineral nutrients and the content was 0.25 mg/100 g,was rich in Mn mineral nutrients and the content was 7.51 mg/100 g. The total amino acid content of Akebia trifoliata was 0.419 mg/100 g,containing 16 kinds of amino acids,including 8 kinds of essential amino acids. The proportion of essential amino acids reached the ideal protein conditions proposed by WHO/FAO,the content of essential amino acids was balanced and the nutritional value was high small amounts of vitamins,fats,proteins and flavonoids. This paper can provide theoretical support and technical guidance for the development and utilization of Akebia trifoliata,and promote the theoretical research process of Akebia trifoliata.