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中国精品科技期刊2020
潘玉婷,杨静,严新宇,等. Fe3O4@ZrO2磁纳米粒子在酪蛋白磷酸肽富集中的应用[J]. 食品工业科技,2021,42(20):223−228. doi: 10.13386/j.issn1002-0306.2020030404.
引用本文: 潘玉婷,杨静,严新宇,等. Fe3O4@ZrO2磁纳米粒子在酪蛋白磷酸肽富集中的应用[J]. 食品工业科技,2021,42(20):223−228. doi: 10.13386/j.issn1002-0306.2020030404.
PAN Yuting, YANG Jing, YAN Xinyu, et al. Application of Fe3O4@ZrO2 Magnetic Nanoparticles in the Selective Enrichment of Casein Phosphopeptides[J]. Science and Technology of Food Industry, 2021, 42(20): 223−228. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020030404.
Citation: PAN Yuting, YANG Jing, YAN Xinyu, et al. Application of Fe3O4@ZrO2 Magnetic Nanoparticles in the Selective Enrichment of Casein Phosphopeptides[J]. Science and Technology of Food Industry, 2021, 42(20): 223−228. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020030404.

Fe3O4@ZrO2磁纳米粒子在酪蛋白磷酸肽富集中的应用

Application of Fe3O4@ZrO2 Magnetic Nanoparticles in the Selective Enrichment of Casein Phosphopeptides

  • 摘要: 本实验将制备好的Fe3O4@ZrO2磁性纳米粒子作为载体,对经胰蛋白酶消化后的酪蛋白磷酸肽(Casein Phosphopeptides,CPP)进行高效地选择性富集。实验以CPP的N/P(摩尔比)以及磁纳米粒子吸附量为评价指标,进行单因素实验以便选取较优的实验因素,分析酪蛋白水解度、吸附pH、吸附时间、吸附温度及肽溶液初始浓度这五个因素对Fe3O4@ZrO2磁性纳米粒子选择性吸附CPP的能力的影响,优化CPP富集的技术参数。结果表明,磁性材料最佳富集工艺系数为:酪蛋白的水解度为22%,反应pH=4.5,吸附温度为30 ℃,吸附时间为50 min,肽溶液初始浓度为50 mg/mL;在此条件下,可得到N/P(摩尔比)为4.87的CPP,磁纳米粒子的吸附量为94.37 mg/g,且用NaOH(pH13)溶液进行解析,CPP的洗脱率可达95%以上。综上,Fe3O4@ZrO2磁性纳米粒子呈现出优异的选择性富集CPP的潜力,对高质量高纯度CPP 的生产具有重要的指导意义。

     

    Abstract: In this study, Fe3O4@ZrO2 magnetic nanoparticles which were synthesized successfully were used as carrier for the highly selective enrichment of phosphopeptides from tryptic digest product of casein . Using N/P (molar ratio) of CPP and magnetic nanoparticles adsorbance as evaluating indicators, the influence of five variables which were the hydrolysis degree of casein, adsorption pH, adsorption time, adsorption temperature, and the initial concentration of phosphopeptides, on the ability of Fe3O4@ZrO2 magnetic nanoparticles to isolate and enrich phosphopeptides was analyzed. The technical parameters of CPP enrichment were optimized. Results showed that, the optimized conditions of CPP enrichment were as below: Casein hydrolysis degree 22%, adsorption pH4.5, adsorption temperature 30 ℃, adsorption time 50 min, the initial concentration of phosphopeptides 50 mg/mL. Finally, under these conditions, the N/P (molar ratio) of CPP was 4.87, and the adsorbance of Fe3O4@ZrO2 magnetic nanoparticles was 94.37. The bound CPP were eluted using NaOH (pH13) and the elution rate was 95% at least. Experiment results demonstrate that ZrO2 coated-magnetic microspheres showed the excellent potential for selective enrichment of CPP, which would play an important role in the production of high quality and purity CPP.

     

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