Abstract: A method was established for the simultaneous determination of 13 sedative residues in aquatic products using modified QuEChERS and high-performance liquid chromatography-tandem mass spectrometry.The samples were extracted with ammoniate acetonitrile,cleaned-up with EMR-Lipid.The analytes were detected in the multi-reaction monitoring(MRM)mode and quantified by the external standard method. The analytes were quantified by matrix-matched standard solutions with external standard method. The linear relationships of 13 sedatives were good and the correlation coefficients(r)were greater than 0.99. The limits of detection(LODs)and the limits of quantification(LOQs)for the analytes were in the range of 0.0103 to 0.1470 μg/kg and 0.0340 to 0.4850 μg/kg. The average recoveries of 13 sedatives spiked at three levels in blank samples ranged from 70.7% to 108.0% with the RSDs from 1.4% to 10.3%.The developed method is easy to operate,sensitive with good purification effect. It can be applied to the rapid determination of the 13 sedatives in aquatic products.