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中国精品科技期刊2020
张旭, 卢娜, 李兆杰, 薛勇, 袁诗涵, 薛长湖, 唐庆娟. 姜黄素光动力对牡蛎脂质氧化水解酶的影响[J]. 食品工业科技, 2020, 41(24): 1-6,12. DOI: 10.13386/j.issn1002-0306.2020030061
引用本文: 张旭, 卢娜, 李兆杰, 薛勇, 袁诗涵, 薛长湖, 唐庆娟. 姜黄素光动力对牡蛎脂质氧化水解酶的影响[J]. 食品工业科技, 2020, 41(24): 1-6,12. DOI: 10.13386/j.issn1002-0306.2020030061
ZHANG Xu, LU Na, LI Zhao-jie, XUE Yong, YUAN Shi-han, XUE Chang-hu, TANG Qing-juan. Effect of Curcumin-Mediated Photodynamic Treatment(PDT) on Lipid Degradation-related Enzymes in Oysters[J]. Science and Technology of Food Industry, 2020, 41(24): 1-6,12. DOI: 10.13386/j.issn1002-0306.2020030061
Citation: ZHANG Xu, LU Na, LI Zhao-jie, XUE Yong, YUAN Shi-han, XUE Chang-hu, TANG Qing-juan. Effect of Curcumin-Mediated Photodynamic Treatment(PDT) on Lipid Degradation-related Enzymes in Oysters[J]. Science and Technology of Food Industry, 2020, 41(24): 1-6,12. DOI: 10.13386/j.issn1002-0306.2020030061

姜黄素光动力对牡蛎脂质氧化水解酶的影响

Effect of Curcumin-Mediated Photodynamic Treatment(PDT) on Lipid Degradation-related Enzymes in Oysters

  • 摘要: 本文探究了姜黄素光动力对牡蛎脂质氧化水解酶的影响,旨在从酶的角度阐明姜黄素光动力减缓牡蛎脂质氧化水解的机理。通过检测牡蛎内源酶活性,筛选新鲜牡蛎中产脂质氧化水解酶的菌株、利用16S rDNA进行菌种鉴定,结合Illumina MiSeq高通量测序技术对新鲜牡蛎中的产酶菌株进行分析。结果表明:姜黄素光动力能够降低内源性脂肪氧合酶(LOX)、脂肪酶(Lipase)、磷脂酶A1(PLA1)、磷脂酶A2(PLA2)、磷脂酶D(PLD)的活性;同时获得了1株分泌脂肪酶的菌—假单胞菌(Pseudomonas)、2株分泌磷脂酶C的菌株—肠杆菌(Enterobacteriaceae)和嗜麦芽寡养单胞菌(Stenotrophomonas);且姜黄素光动力能够减少这些菌株的数量(P<0.05),从而减少外源酶的数量。因此,姜黄素光动力可以通过降低牡蛎内源酶活性、减少外源酶数量来减缓牡蛎脂质的氧化水解。

     

    Abstract: In this paper,the effects of curcumin-mediated photodynamic treatment(PDT)on lipid degradation-related enzymes in oysters were investigated,aimed to elucidate the mechanism of curcumin-mediated PDT delaying lipids oxidative and hydrolysis from the perspective of enzymes. By detecting the activity of endogenous enzymes,selecting the strains secreted enzyme,identifying strain used 16S rDNA,analyzing enzyme-producing strains used Illumina MiSeq high-throughput sequencing technology in fresh oysters. The results showed that curcumin-mediated PDT could reduce the activity of endogenous lipoxygenase(LOX),phospholipase A1(PLA1),phospholipase A2(PLA2),and phospholipase D(PLD). One strains secreted lipases(Pseudomonas),two strains secreted phospholipase C(Enterobacteriaceae and Stenotrophomonas)were selected. What’s more,curcumin-mediated PDT could reduce the number of those bacteria secreted enzyme(P<0.05),thus reducing the number of exogenous enzymes. Therefore,curcumin-mediated PDT could delay the oxidative and hydrolysis of lipids by reducing the activity of endogenous enzymes and the amount of exogenous enzymes in oysters.

     

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