Abstract: This paper demonstrated a fast approach to test 6 kinds of fluoroquinolones(enrofloxacin,ciprofloxacin,ofloxacin,norfloxacin,lomefloxacin,pefloxacin)in bean sprouts by UPLC-MS/MS method. The sample was firstly extracted by 1% formic acid acetonitrile,the extraction solution was purified by dispersed solid phase extraction using MgSO₄,PSA,C₁₈,GCB. A gradient separation was performed on the supernatant via ACQUITY UPLC BEH C₁₈ column(mobile phase:methanol and 0.1% formic acid). The separated subjects were tested by UPLC-MS using MS Multi-Reaction-Monitor(MRM)technique,and quantified using internal standard method. The results indicated that the correlation between concentration of fluoroquinolone and signal was linear when the concentration was in the range of 1.0~100.0 μg/L. The correlation coefficient was larger than 0.9982.The limits of detection were within 0.1~0.3 μg/kg,and the limits of quantitation were within 0.3~1.0 μg/kg. The results indicated the rates were between 96.53%~106.94% when the subject concentration was 2,4 and 20 μg/kg,separately. The relative standard deviations were in the range of 2.73%~7.60%. The method is easy,fast,sensitive,accurate and stable. It is suitable for simultaneous detection of 6 kinds of fluoroquinolones in bean sprouts.