Abstract: In order to study the stability(the thermal,light and pH stability)of verbascoside,this paper discussed the effects of temperature(37,50,60,80,100 ℃),pH(2,6,7,8)and light on the degradation kinetics of verbascoside by HPLC. The results showed that the degradation reaction of verbascoside in different temperature,pH and light/dark conditions belonged to the first order reaction. The results showed that high temperature(above 60 ℃),near neutral environment(pH6~8)and light were not conducive to the preservation of verbascoside. The half-life of verbascoside was more stable at medium low temperature(37,50 ℃),low pH(2)and dark conditions,and the half-life was significantly prolonged. In this paper,the main factors affecting the stability of verbascoside were analyzed,which could provide an important reference for the extraction,purification and activity analysis of verbascoside.