Abstract: Objective:To study the laxative and regulae function of black jerusalem artichoke water extract on constipation mice induced by compound diphenoxylate. Mice were randomly divided into 9 groups,namely control check treatment(CK),model control treatment(MC),active control treatment(AC),low,medium and high dose treatment of raw jerusalem artichoke,and low,medium and high dose treatment of black jerusalem artichoke,the doses corresponding to the low,medium and high dose treatment were 2.5,5.0 and 10.0 g/kg bw,respectively. Then the changes of body weight,intestinal transit,defecation situation,and the contents of short-chain fatty acids in feces of mice were measured. Results:The results showed that compared with mice in CK,the time of first melena of mice in MC was significantly delayed(P<0.05),the ink propelling rate,the number and mass of defecation particles within six hours were significantly reduced(P<0.05),and the total SCFAs in feces was significantly reduced(P<0.01),indicating that the constipation model was successfully established. In comparison with mice in MC,the mice fed on black jerusalem artichoke extract of 5.0 and 10.0 g/kg bw could significantly shorten the time of first melena by 24.57% and 36.56%,increase the number of melena by 67.64% and 85.29% and the quality of melena by 87.31% and 67.26%,increase the ink propelling rate by 70.59% and 101.02%,respectively.Furthermore,the amount of short-chain fatty acids in the intestinal,such as acetic acid,propionic acid and butyric acid were increased. Conclusion:5.0 and 10.0 g/kg bw black jerusalem artichoke extract can effectively promote constipated mice intestinal wriggling,improve intestinal environment and alleviate constipation,and has the function of laxative and regulating.This study provides data support for the health benefits of black jerusalem artichoke.