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中国精品科技期刊2020
赵桉, 李欣芮, 范小飘, 高文文, 尚佳萃, 万峰, 孟祥晨. 婴儿源双歧杆菌对人胎结肠上皮细胞的增殖作用及机制研究[J]. 食品工业科技, 2020, 41(20): 307-313. DOI: 10.13386/j.issn1002-0306.2020.20.051
引用本文: 赵桉, 李欣芮, 范小飘, 高文文, 尚佳萃, 万峰, 孟祥晨. 婴儿源双歧杆菌对人胎结肠上皮细胞的增殖作用及机制研究[J]. 食品工业科技, 2020, 41(20): 307-313. DOI: 10.13386/j.issn1002-0306.2020.20.051
ZHAO An, LI Xin-rui, FAN Xiao-piao, GAO Wen-wen, SHANG Jia-cui, WAN Feng, MENG Xiang-chen. Proliferation Effect and Mechanism of Bifidobacterium from Infantis on Human Fetal Colon Epithelial Cells[J]. Science and Technology of Food Industry, 2020, 41(20): 307-313. DOI: 10.13386/j.issn1002-0306.2020.20.051
Citation: ZHAO An, LI Xin-rui, FAN Xiao-piao, GAO Wen-wen, SHANG Jia-cui, WAN Feng, MENG Xiang-chen. Proliferation Effect and Mechanism of Bifidobacterium from Infantis on Human Fetal Colon Epithelial Cells[J]. Science and Technology of Food Industry, 2020, 41(20): 307-313. DOI: 10.13386/j.issn1002-0306.2020.20.051

婴儿源双歧杆菌对人胎结肠上皮细胞的增殖作用及机制研究

Proliferation Effect and Mechanism of Bifidobacterium from Infantis on Human Fetal Colon Epithelial Cells

  • 摘要: 分析12株婴儿源双歧杆菌的发酵上清液和破碎物对人胎结肠上皮细胞(CCD841 CoN)的增殖效果。筛选出对细胞增殖促进效果最好的菌株,提取其表面蛋白和分泌蛋白,检测这两类蛋白对细胞增殖、周期及增殖相关基因表达的影响,探讨其对CCD841 CoN细胞增殖促进的作用。结果表明:筛选出双歧杆菌H3-R2,其浓度为50 μg/mL表面蛋白和10 μg/mL分泌蛋白与CCD841 CoN细胞共作24 h后,对细胞有显著增殖促进作用(P<0.05),提高细胞S期比例,降低G0/G1期比例(P<0.05),提高细胞内 β-catenin、c-Myc和Cyclin D1基因mRNA的转录水平(P<0.05),降低 Axin2、GSK-3β 基因mRNA的转录水平(P<0.05)。综上,婴儿源双歧杆菌可在mRNA转录水平上对人胎结肠上皮细胞有增殖促进作用,具有促进新生儿肠道发育的潜在应用价值。

     

    Abstract: The proliferative effects of fermented supernatants and crushed matter from 12 infant-derived Bifidobacterium on human fetal colon epithelial cells(CCD841 CoN)were analyzed. The bifidobacterial with the best pro-proliferative effect were screened out,and their surface and secreted proteins were extracted. The effects of these two types of proteins on cell proliferation,cell cycle,and proliferation-related genes expression were examined,and the role of CCD841 CoN cell proliferation was explored. The results showed that Bifidobacterium bifidum H3-R2 was screened,and it contained 50 μg/mL of surface protein and 10 μg/mL of secreted protein. After incubation with CCD841 CoN cells for 24 h,it had a significant proliferation promotion effect on the cells(P<0.05)and increased cell S-phase ratio,decreased G0/G1 phase ratio(P<0.05),increased intracellular β-catenin,c-Myc and Cyclin D1 gene mRNA transcription levels(P<0.05),decreased Axin2,GSK-3β gene mRNA transcription level(P<0.05). In summary,infant-derived bifidobacterial could promote the proliferation of human fetal colon epithelial cells at the level of mRNA transcription,and had potential application value in promoting the intestinal development of newborns.

     

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