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中国精品科技期刊2020
鲍佳彤, 杨淇越, 宁云霞, 梁丽雅, 李玲, 马俪珍. 非肌肉蛋白对未漂洗革胡子鲶鱼鱼糜凝胶特性的影响[J]. 食品工业科技, 2020, 41(20): 46-53,71. DOI: 10.13386/j.issn1002-0306.2020.20.008
引用本文: 鲍佳彤, 杨淇越, 宁云霞, 梁丽雅, 李玲, 马俪珍. 非肌肉蛋白对未漂洗革胡子鲶鱼鱼糜凝胶特性的影响[J]. 食品工业科技, 2020, 41(20): 46-53,71. DOI: 10.13386/j.issn1002-0306.2020.20.008
BAO Jia-tong, YANG Qi-yue, NING Yun-xia, LIANG Li-ya, LI Ling, MA Li-zhen. Effects of Non-muscle Protein on Gel Properties of Unwashed Clarias gariepinus surimi[J]. Science and Technology of Food Industry, 2020, 41(20): 46-53,71. DOI: 10.13386/j.issn1002-0306.2020.20.008
Citation: BAO Jia-tong, YANG Qi-yue, NING Yun-xia, LIANG Li-ya, LI Ling, MA Li-zhen. Effects of Non-muscle Protein on Gel Properties of Unwashed Clarias gariepinus surimi[J]. Science and Technology of Food Industry, 2020, 41(20): 46-53,71. DOI: 10.13386/j.issn1002-0306.2020.20.008

非肌肉蛋白对未漂洗革胡子鲶鱼鱼糜凝胶特性的影响

Effects of Non-muscle Protein on Gel Properties of Unwashed Clarias gariepinus surimi

  • 摘要: 为进一步提高未经漂洗革胡子鲶鱼(Clarias gariepinus,CG)鱼糜的凝胶特性,本试验在CG鱼糜中添加0.4%转谷氨酰胺酶和20 mmol/kg CaCl2基础上(CK组),再依次添加不同浓度的大豆分离蛋白(soybean protein isolation,SPI)、蛋清蛋白(egg white protein,EWP)和浓缩乳清蛋白(whey protein concentrate,WPC),SPI添加量分别为CG鱼糜肉重的2%(SPIL)、5%(SPIM)和7%(SPIH),EWP添加量分别记为EWPL(0.1%)、EWPM(0.3%)和EWPH(0.5%),WPC记为WPCL(0.2%)、WPCM(0.4%)和WPCH(0.6%),共制备出10组CG鱼糜凝胶。研究非肌肉蛋白对CG鱼糜的凝胶特性、色泽、持水力、动态流变学性质和微观结构等品质特性的影响。结果表明:整体上非肌肉蛋白的添加会提高CG鱼糜的凝胶特性和TGase活力。其中,SPIL、EWPH和WPCL三组的CG鱼糜凝胶表现出较好的凝胶强度,依次为3322.46、3555.90和4149.31 g·mm,并且网络结构较其他组更紧密、均匀;EWP和WPC的三种添加浓度均会提高CG鱼糜凝胶的白度值;SPI均可使CG鱼糜凝胶的T22向弛豫时间短的方向迁移,其中SPIH组的凝胶持水性(86.64%)最高。动态流变结果表明,三种非肌肉蛋白的添加对CG鱼糜的弹性模量G'和损耗模量G″均产生显著影响,SPIH组、EWPM组和WPCL组为组内最高值。综上分析,在CG鱼糜凝胶中分别添加鱼糜总量的2% SPI、0.5% EWP、0.2% WPC,可显著提高CG鱼糜的凝胶特性。

     

    Abstract: In order to further enhance the gel properties of Clarias gariepinus(CG)surimi unwashed. In this experiment,on the basis of adding 0.4% transglutaminase and 20 mmol/kg CaCl2 to CG surimi(CK group),different concentrations of soy protein isolation(SPI),egg white protein(EWP)and whey protein concentrate(WPC)were added successively. The amount of SPI added was 2%(SPIL),5%(SPIM),and 7%(SPIH)of CG surimi meat. The amount of EWP added was recorded as EWPL(0.1%),EWPM(0.3%),and EWPH(0.5%). WPC is recorded as WPCL(0.2%),WPCM(0.4%),and WPCH(0.6%). A total of 10 groups of CG surimi gels were prepared. The effects of these non-muscle proteins on the gel properties,color,water holding capacity,dynamic rheological properties and microstructure of CG surimi were studied. The results showed that the addition of non-muscle protein increased the gel properties and TGase activity of CG surimi. Compared with each group,CG surimi gels of SPIL,EWPH and WPCL showed higher gel strength,which was 3322.46,3555.90 and 4149.31 g·mm,and the network structure was closer and more uniform than that of other groups. The concentration of EWP and WPC increased the whiteness of CG surimi gel by adding three concentrations. SPI could make the T22 of CG surimi gel migrate to the direction of short relaxation time,and gel water holding capacity(86.64%)of SPIH group were the highest. The dynamic rheological results also showed that the three non-muscle proteins had a significant effect on the elastic modulus G' and loss modulus G″ of CG surimi,and the SPIH,EWPM and WPCL groups were the maximum values in each group. In summary,the gel characteristics of CG surimi were significantly increased by adding 2% SPI,0.5% EWP and 0.2% WPC in surimi gel respectively in CG surimi gel.

     

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