Abstract: In order to study the effects of different storage temperatures on the texture, tissue structure, endogenous protease activity and water characteristics of Sipunculus nudus (SN) and Phascolosoma esculenta (PE), in this study, fresh SN and PE were used as raw materials and stored at 4 and 30℃ for 48 and 22 h, respectively. The texture characteristics, fiber structure, endogenous protease activity, and water characteristics were used as indicators. The results showed that:Storage at 4℃ for 0~16 h and 30℃ for~10 h, SN's hardness and chewiness decreased by 19.08%, 33.94% and 41.61%, 41.39% respectively, PE's hardness and chewiness decreased by 16.06%, 12.62% and 25.02%, 17.68%, respectively, while other indicators did not change significantly. By contrast, storage at 4℃ for 16~24 h and 30℃ for 10~14 h, texture size, endogenous protease activity, water activity, the proportion of free water and non-flowable water had a significant change (P<0.05), the fiber was bent, and the gap increased. Conclusion:The degree of changes in all indicators of the sipunculids during storage at 4℃ was less than 30℃.The storage at 4℃ and 30℃ should be controlled within 0~16 h and 0~10 h, respectively.