Abstract: To investigate the effects of Gardenia flower pectin and apple pectin on the release of eugenol in liposomes. In this paper, the preparation conditions of eugenol liposome were studied and the concentration of gardenia flower pectin and apple pectin for its coating was optimized. Finally, the stability of liposome at different temperatures, sucrose concentrations and sodium chloride concentrations were studied. And the influence of pectin coating on eugenol release at pH1.2 and pH7.4 was investigated. The results showed that the preparation condition of eugenol liposome (EP) was 1.5% lecithin, 0.5% tween 20 and 20% ethanol. Under these condition, the optimal concentrations of the two pectin coatings were 0.2%. Compared with EP, the particle size of GLMP-EP and ALMP-EP were increased 9.0 and 21.2 nm, but the zeta potential values dropped to -32.6 and -19.8 mV, respectively, which indicated the stability improved. And TEM result showed that the pectin successfully coated on the surface of liposomes. EP, GLMP-EP and ALMP-EP showed good stability after a storage of 60 days at different temperatures (4 and 25℃), sucrose (0, 50和100 mg/mL) and sodium chloride (0, 50和100 mmol/L), the encaopsulation efficiency was all over 70%. The in vitro release indicated that GLMP-EP could delay the release of eugenol at pH1.2 and pH7.4, but ALMP-EP could only delay the release of eugenol at pH1.2, which indicated that GLMP-EP had better stability under different pH conditions than that of ALMP-EP. The data suggested that the coating of gardenia flower pectin and apple pectin did not impair the storage stability of eugenol liposomes, but the coating of gardenia flower pectin could delay the release of eugenol in liposomes.