Abstract: In this work,reduced glutathione nano-liposomes(Lip)were prepared by thin-film ultrasonic method and double-modified with both chitosan and sodium alginate. Then the storage stability and digestion properties in gastrointestinal tract of Lip with or without modification were measured. Additionally,the release kinetics of glutathione in gastrointestinal tract was also analyzed through the usage of different models. The results showed that Lip modified with both sodium alginate and chitosan(AL-CH-Lip)had a spherical shape,an average size of(94.44±1.71) nm,and a Zeta potential of(-42.57±0.91) mV. Moreover,the loading efficiency of AL-CH-Lip was measured to be 93.5%±0.16%. Compared with unmodified or chitosan modified Lip,AL-CH-Lip had higher storage stability and digestion stability,and was more suitable for application in food industries and medical areas.