Abstract: In this study,the squid visceral crude oil was refined by deacidification with alkali and decolorization with activated clay. Taking acid value and decolorization rate as the evaluation index of deacidification efficiency and decolorization efficiency respectively,central composite design based on single factor experiments was applied to optimize the refining process. Moreover,the relative physicochemical indexes of squid viscera oil before and after refining were analyzed and determined. The results showed that the optimal conditions for deacidification were alkali concentration 26 °Bé,temperature 54℃,time 26 min,which resulted in that the acid value of squid visceral oil reached(0.63±0.05) mg/g. The optimal conditions for decolorization were dosage of activated clay 3.7%,temperature 88℃,time 60 min,which resulted in that the decolorization rate of squid visceral oil reached 92.38%±1.25%. After the treatment of refining process,the acid value and color of squid visceral oil reached the quality criterion of first class refined fish oil,and the other physicochemical indexes reached or were close to the quality criterion of secondary refined fish oil. The contents of polyunsaturated fatty acid,EPA and DHA were 38.91%,12.56% and 16.32%,respectively. The above results indicate that squid viscera crude oil can be efficiently refined by the process optimized in this study,which can be used in commercial production.