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中国精品科技期刊2020
周月, 付敏, 刘欢. 茶氨酸联合γ-氨基丁酸改善CUMS大鼠抑郁症状的研究[J]. 食品工业科技, 2020, 41(2): 292-297,306. DOI: 10.13386/j.issn1002-0306.2020.02.047
引用本文: 周月, 付敏, 刘欢. 茶氨酸联合γ-氨基丁酸改善CUMS大鼠抑郁症状的研究[J]. 食品工业科技, 2020, 41(2): 292-297,306. DOI: 10.13386/j.issn1002-0306.2020.02.047
ZHOU Yue, FU Min, LIU Huan. The Ameliorative Effect of L-theanine Combined with γ-aminobutyric Acid on CUMS-induced Depression in Rats[J]. Science and Technology of Food Industry, 2020, 41(2): 292-297,306. DOI: 10.13386/j.issn1002-0306.2020.02.047
Citation: ZHOU Yue, FU Min, LIU Huan. The Ameliorative Effect of L-theanine Combined with γ-aminobutyric Acid on CUMS-induced Depression in Rats[J]. Science and Technology of Food Industry, 2020, 41(2): 292-297,306. DOI: 10.13386/j.issn1002-0306.2020.02.047

茶氨酸联合γ-氨基丁酸改善CUMS大鼠抑郁症状的研究

The Ameliorative Effect of L-theanine Combined with γ-aminobutyric Acid on CUMS-induced Depression in Rats

  • 摘要: 目的:观察L-茶氨酸和γ-氨基丁酸(γ-aminobutyric acid,GABA)联合干预对慢性不可预知温和刺激(CUMS)模型大鼠抑郁症状的缓解作用。方法:采用CUMS法建立抑郁症大鼠模型,不同剂量L-茶氨酸和GABA混合物或西酞普兰连续灌胃45 d,期间持续CUMS刺激。另设对照组,常规饲养。监测大鼠体重变化、糖水偏好程度、以及旷场实验水平运动、垂直运动等行为学指标,测定大鼠血清及脑组织中五羟色胺(5-HT)、多巴胺(DA)、白介素-6(IL-6)、脑源性神经营养因子(BDNF)、β-内啡肽(β-EP)的水平。结果:与对照组比较,CUMS大鼠体重、糖水消耗百分比、旷场水平和垂直运动显著降低(P<0.05);与模型组比较,茶氨酸联合GABA干预可显著增加大鼠体重增量、糖水消耗百分比,运动距离和站立次数(P<0.05),抑郁大鼠血清及脑组织中5-HT、DA以及BDNF、β-EP的含量增加(P<0.05),降低IL-6的水平(P<0.05)。结论:L-茶氨酸和GABA联合干预对CUMS诱发的抑郁症有一定改善作用,且高剂量的作用更为显著。

     

    Abstract: Objective:To observe the relaxation effect of combined intervention of L-theanine and γ-aminobutyric acid(GABA)on chronic unpredictable mild stress(CUMS)rats. Methods:The rat model of depression was established by CUMS. A mixture of L-theanine and GABA or citalopram with different dose was administered orally for 45 days during continuous CUMS stimulation.A control group was set up and fed routinely. The changes of body weight,sugar preference,horizontal and vertical movement were monitored. The levels of serotonin(5-HT),dopamine(DA),interleukin-6(IL-6),brain-derived neurotrophic factor(BDNF)and β-endorphin(β-EP)in serum and brain tissue were measured. Results:Compared with the control group,the body weight,percentage of sugar consumption,horizontal and vertical movement of CUMS rats decreased significantly(P<0.05). Compared with the model group,L-theanine combined with GABA could significantly increase body weight,sugar consumption percentage,horizontal movement distance and vertical movement frequency(P<0.05),enhance the contents of 5-HT,DA,BDNF and β-EP and decrease the level of IL-6 in brain tissue and serum of depressive rats(P<0.05). Conclusion:The combined intervention of L-theanine and GABA can improve the depression induced by CUMS,and the effect of high dose is more significant.

     

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