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中国精品科技期刊2020
马雁, 沈桂奇, 陶志强, 王丽, 黄欣媛, 苏剑波, 伍云, 张兆俊, 印伯星, 关成冉. 贮藏温度对真空冻干发酵剂发酵乳杆菌grx07活性的影响[J]. 食品工业科技, 2020, 41(2): 84-88,94. DOI: 10.13386/j.issn1002-0306.2020.02.014
引用本文: 马雁, 沈桂奇, 陶志强, 王丽, 黄欣媛, 苏剑波, 伍云, 张兆俊, 印伯星, 关成冉. 贮藏温度对真空冻干发酵剂发酵乳杆菌grx07活性的影响[J]. 食品工业科技, 2020, 41(2): 84-88,94. DOI: 10.13386/j.issn1002-0306.2020.02.014
MA Yan, SHEN Gui-qi, TAO Zhi-qiang, WANG Li, HUANG Xin-yuan, SU Jian-bo, WU Yun, ZHANG Zhao-jun, YIN Bo-xing, GUAN Cheng-ran. Effects of Storage Temperature on the Viability of Lactobacillus fermentum grx07 under Vacuum Freeze-dried Start[J]. Science and Technology of Food Industry, 2020, 41(2): 84-88,94. DOI: 10.13386/j.issn1002-0306.2020.02.014
Citation: MA Yan, SHEN Gui-qi, TAO Zhi-qiang, WANG Li, HUANG Xin-yuan, SU Jian-bo, WU Yun, ZHANG Zhao-jun, YIN Bo-xing, GUAN Cheng-ran. Effects of Storage Temperature on the Viability of Lactobacillus fermentum grx07 under Vacuum Freeze-dried Start[J]. Science and Technology of Food Industry, 2020, 41(2): 84-88,94. DOI: 10.13386/j.issn1002-0306.2020.02.014

贮藏温度对真空冻干发酵剂发酵乳杆菌grx07活性的影响

Effects of Storage Temperature on the Viability of Lactobacillus fermentum grx07 under Vacuum Freeze-dried Start

  • 摘要: 以真空冻干的发酵剂grx07为研究对象,通过测定该发酵剂在20、4、-20℃三种温度下贮藏的存活率、活菌数、水分活度、发酵活力及影响乳酸菌代谢活力的关键酶活(乳酸脱氢酶、β-半乳糖苷酶、ATP酶的酶活),来探讨不同的贮藏温度对发酵乳杆菌grx07菌粉活力的影响。结果表明,发酵剂grx07在20℃贮藏3个月时存活率低于1%;在4和-20℃保藏12个月时菌粉存活率分别为4.45%和51.05%。在-20℃贮藏12个月的活菌数仍能达到3.78×1011 cfu/g,菌粉水分活度为0.210;活化后发酵pH达到4.30,乳酸脱氢酶、β-半乳糖苷酶、ATP酶的活力分别为贮藏前酶活力的80.24%、74.60%、87.10%。因此,真空冻干发酵剂grx07长期贮藏的适宜温度为-20℃。

     

    Abstract: To investigate the effect of different storage temperatures on the viability of vacuum freeze-dried fermenting agent Lactobacillus fermentum grx07,the survival rate,viable cell count,water activity,fermentation activity and the key enzyme activities of vitality related(lactate dehydrogenase,β-galactosidase,ATPase)were measured at 20,4 and -20℃,respectively. The results showed that the three-month survival rate of starter grx07 was less than 1% at 20℃,while the survival rates was seperately 4.45% and 51.05% at 4 and -20℃ for 12 months. The number of viable cells stored at -20℃ for 12 months were still reached to 3.78×1011 cfu/g,the water activity of the powder was 0.210,and the fermentation pH was 4.30. Moreover,the activities of lactate dehydrogenase,β-galactosidase,and ATPase were separately 80.24%,74.60%,87.10% of that prior to the storage. Therefore,the suitable temperature for long-term storage of the vacuum freeze-dried starter grx07 was -20℃.

     

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