Abstract: In this study,phytosterol(PS)and phosphatidylcholine(PC)were used as materials to prepare protein-modified phytosterol liposomes(SPI-LS)by ethanol injection method. Particle size,PdI,Zata potential,and the embedding rate were used to evaluate the technology conditions of preparation liposome by single factor optimization. The solubility of SPI-LS in water was observed. The bioactivity of digestion in vitro before and after PS embedding were investigated. The results showed that the optimum and technological condition were as follows:The ratio of PS to PC 1:4,ethanol volume ratio 25%,and protein composed 1%. There was no significant difference in the appearances of liposomes after solubilization and before lyophilization,and the solubility of PS was up to 1.971 mg/mL. After 3 h in vitro gastrointestinal digestion,the bioaccessibility of PS in SPI-LS could be maintained more than 50%,which was three times higher than that of free PS.Therefore,the most optimal SPI-LS had good solubility,and could effectively maintain the biological activity of phytosterols.