• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
杨玲, 刘成浩, 韩萧茜, 刘齐, 冯曼琳, 杜勇. 对一测多评法测定蜂胶类保健食品中芹菜素等5种成分含量的评价研究[J]. 食品工业科技, 2020, 41(1): 229-235,251. DOI: 10.13386/j.issn1002-0306.2020.01.037
引用本文: 杨玲, 刘成浩, 韩萧茜, 刘齐, 冯曼琳, 杜勇. 对一测多评法测定蜂胶类保健食品中芹菜素等5种成分含量的评价研究[J]. 食品工业科技, 2020, 41(1): 229-235,251. DOI: 10.13386/j.issn1002-0306.2020.01.037
YANG Ling, LIU Cheng-hao, HAN Xiao-qian, LIU Qi, FENG Man-lin, DU Yong. Study on Determination of Apigenin and Other Five Components in Propolis Health Foods by Quantitative Analysis of Multi-components by Single-maker[J]. Science and Technology of Food Industry, 2020, 41(1): 229-235,251. DOI: 10.13386/j.issn1002-0306.2020.01.037
Citation: YANG Ling, LIU Cheng-hao, HAN Xiao-qian, LIU Qi, FENG Man-lin, DU Yong. Study on Determination of Apigenin and Other Five Components in Propolis Health Foods by Quantitative Analysis of Multi-components by Single-maker[J]. Science and Technology of Food Industry, 2020, 41(1): 229-235,251. DOI: 10.13386/j.issn1002-0306.2020.01.037

对一测多评法测定蜂胶类保健食品中芹菜素等5种成分含量的评价研究

Study on Determination of Apigenin and Other Five Components in Propolis Health Foods by Quantitative Analysis of Multi-components by Single-maker

  • 摘要: 目的:建立以蜂胶为单一原料的保健食品中芹菜素、山奈酚、白杨素、松属素及高良姜素的"一测多评"含量测定方法。方法:采用高效液相色谱法,以芹菜素为参照物,建立其与山奈酚、白杨素、松属素、高良姜素的相对校正因子,利用相对校正因子计算5种成分的含量,实现一测多评;并对多批样品同时采用标准曲线法测定,将结果进行比较,验证方法的可行性。结果:5种成分在相应的范围内线性关系良好,相关系数r>0.9999,平均加标回收率在96.54%~102.06%,RSD在1.4%~2.8%,山奈酚、白杨素、松属素、高良姜素的相对校正因子分别为1.1929、0.5938、2.7847、0.9727,且在不同实验条件下重现性良好,一测多评法计算值与标准曲线法实测值相对平均偏差在0.5%~2.1%之间,测定结果基本一致。结论:本文建立的一测多评方法操作简便,重复性好,可为以蜂胶为单一原料的保健食品的质量评价提供参考。

     

    Abstract: Objective:To establish the "quantitative analysis of multi-components by single-marker(QAMS)"method for the determination of apigenin,kaempferol,chrysin,pinocembrin and galangin in health food with propolis as a single raw material. Methods:High performance liquid chromatography was used and apigenin as reference substance to establish its relative correction factors with kaempferol,chrysin,pinocembrin and galangin. Using relative correction factor to calculate the content of five components to realize"QAMS". At the same time,the method of standard curve was used to compare the results and verify the feasibility of the method. Results:Within a certain linear range,5 components had good linear relationships,r>0.9999.The average recovery rate of 5 components were in the range of 96.54%~102.06%,and the relative standard deviations(RSDs)were in the range of 1.4%~2.8%. The relative correction factors(RCFs)of kaempferol,chrysin,pinocembrin and galangin with reference to isoferulic acid were 1.1929,0.5938,2.7847,0.9727,respectively. The relative correction factors of each component had good reproducibility under different experimental conditions. There was almost no difference between the calculated values of "QAMS" method and the measured values of standard curve method and the relative average deviations were in the range of 0.5%~2.1%. Conclusion:The method of "QAMS" is simple and reproducible,and can provide reference for the quality evaluation of health food with propolis as a single raw material.

     

/

返回文章
返回