Abstract: In order to compare the differences between prebiotics and the laxative function,168 male Kunming mice were randomly divided into two laboratory groups,one for small intestine movement experiment and the other for defecation experiment. Each trail group was divided into blank control group,model control group,stachyose group,fructooligosaccharides group,galactooligosaccharide group,polydextrose group,inulin group. The interventiondose of five prebiotics was 1.0 g/kg. After 14 days of intervention,loperamide was used to establish the constipation model of mice,then the intestinal ink propulsion rate,first black stool time,fecal number and fecal weight within 6 h were measured.The results of small intestine movement experiment showed that,compared with the model control group,the intestinal ink propulsion rate was significantly increased in the groups of stachyose,fructooligosaccharides,galactooligosaccharide and inulin(P<0.05). The intestinal ink propulsion rate of polydextrose increased,but the differences had not significant. The result of defecation experiment showed that no significant differences in the first defecation time,the fecal number and fecal weight within 6 h of the five prebiotics(P>0.05). When the intervention dose was 1.0 g/kg,all the tachyose,fructooligosaccharides,galactose oligogalactose and inulin had a promoting effects on intestinal movement,but had no effect on defecation.There was no significant difference in the effect of different prebiotics on intestinal motility.