Abstract: In order to obtain crystallized D-psicose,a crystallization process of D-psicose in ethanol system was carried out. The effects of four main influencing factors(including density of D-psicose solution,the ratio of ethanol to D-psicose,crystallizing time and temperature)on recovery rate of D-psicose were tested. Based on these single factor experiments,response surface methodology was used to optimize crystallizing process. Results illustrated that the optimal operational conditions were as follow:Density of D-psicose solution was 1.35 g/mL,ratio of ethanol to D-psicose was 3.8:1,crystallizing time and temperature were 325 min and 25 ℃. Under these conditions,the recovery rate was 71.58%. The above results illustrated that crystallized D-psicose could be obtained in ethanol system. Besides,the models obtained in this study could be used to optimize the crystallizing process of D-prisose in ethanol system.