Abstract: Based on the single factor experiment,chickpea was chosen as raw material and the response surface analysis method was applied to optimize the process conditions of ultrasonic assisted extraction of phytosterols. The column chromatography was used for purification,GC-MS was utilized to analyze the components after purification and its antimicrobial activity was further evaluated. Results showed that,the optimization were as follows:Ultrasonic power 450 W,extraction time 7 min,and the ratio of solvent to material 10:1 mL/g. Under these conditions,the actual yield was(57.99±3.37) mg/100 g·dw. The recovery rate of phytosterols by column chromatography was 53.44%. After identification of GC-MS,the phytosterols in the chickpeas were mainly β-sitosterol(80.43%),campesterol(12.48%)and stigmasterol(7.09%). The results of bacteriostatic experiments showed that the purified phytosterols had a dose-effect relationship with Escherichia coli,Bacillus subtilis,Staphylococcus aureus and Salmonella. This study provides a reference for further development and utilization of phytosterols in chickpeas.