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中国精品科技期刊2020
刘晓飞, 程传兴, 宋洁, 郑志辉, 南雪梅, 关桦楠, 张娜. 发芽时间对糙米糖蛋白抗氧化能力的影响[J]. 食品工业科技, 2019, 40(20): 16-22,28. DOI: 10.13386/j.issn1002-0306.2019.20.003
引用本文: 刘晓飞, 程传兴, 宋洁, 郑志辉, 南雪梅, 关桦楠, 张娜. 发芽时间对糙米糖蛋白抗氧化能力的影响[J]. 食品工业科技, 2019, 40(20): 16-22,28. DOI: 10.13386/j.issn1002-0306.2019.20.003
LIU Xiao-fei, CHENG Chuan-xing, SONG Jie, ZHENG Zhi-hui, NAN Xue-mei, GUAN Hua-nan, ZHANG Na. Effect of Germination Time on Antioxidant Capacity of Brown Rice Glycoprotein[J]. Science and Technology of Food Industry, 2019, 40(20): 16-22,28. DOI: 10.13386/j.issn1002-0306.2019.20.003
Citation: LIU Xiao-fei, CHENG Chuan-xing, SONG Jie, ZHENG Zhi-hui, NAN Xue-mei, GUAN Hua-nan, ZHANG Na. Effect of Germination Time on Antioxidant Capacity of Brown Rice Glycoprotein[J]. Science and Technology of Food Industry, 2019, 40(20): 16-22,28. DOI: 10.13386/j.issn1002-0306.2019.20.003

发芽时间对糙米糖蛋白抗氧化能力的影响

Effect of Germination Time on Antioxidant Capacity of Brown Rice Glycoprotein

  • 摘要: 为了明确发芽时间对糙米糖蛋白含量及抗氧化性能的影响,本研究将糙米分别发芽12、24、36、48、60、72 h、84、96 h,考察发芽糙米中的糖蛋白对DPPH自由基、超氧阴离子自由基、羟自由基的清除能力、铁离子还原能力和抗亚油酸氧化能力的影响,以抗坏血酸为阳性对照,研究发芽时间和糙米糖蛋白浓度与抗氧化能力的相关性。结果发现,糙米发芽84 h时,4 mg/mL的糖蛋白有最佳的抗氧化能力。此时,糖蛋白对羟自由基和超氧阴离子自由基的清除能力优于抗坏血酸,清除率分别为99.49%和99.39%;对DPPH自由基清除能力、铁离子还原能力和抗亚油酸能力稍弱于抗坏血酸,清除率为88.11%,吸光度值分别为2.06和0.211。糙米糖蛋白的抗氧化能力受发芽时间和样液浓度的影响,发芽时间与DPPH自由基、羟自由基的清除率和抗亚油酸氧化能力呈极显著正相关(P<0.01),与铁离子还原能力呈显著正相关(P<0.05);糖蛋白浓度与超氧阴离子清除率,铁离子还原能力和抗亚油酸氧化呈极显著正相关(P<0.01)。

     

    Abstract: In order to clarify the effects of germination time on brown rice glycoprotein contents and its antioxidant properties,this study germinated brown rice for 12,24,36,48,60,72,84,96 h,respectively. The effects of germinated brown rice glycoprotein on DPPH radical,superoxide anion radical,hydroxyl radical scavenging ability,iron ion reduction ability and resistance to linoleic acid oxidation were investigated. Ascorbic acid was used as positive control. The relationship between germinating time and brown rice glycoprotein concentration or antioxidant ability were studied. Results showed that,the brown rice glycoprotein germinated for 84 h had the best antioxidant ability at the concentration of 4 mg/mL,and the scavenging ability of hydroxyl radical and superoxide anion radical was better than that of ascorbic acid,and the clearance rates were 99.49% and 99.39%,respectively. The scavenging ability of DPPH free radical,the ability of iron ion reduction and the ability of resisting linoleic acid were slightly weaker than those of ascorbic acid,the clearance rate was 88.11%,and the absorbance values were 2.06 and 0.211,respectively. The antioxidant capacity of brown rice glycoprotein was affected by germination time and concentration of sample. The germination time was significantly positively correlated with DPPH free radical,hydroxyl radical scavenging rate and anti-oil oxidizing ability(P<0.01),and iron ion. The reducing ability was significantly positively correlated(P<0.05). The glycoprotein concentration was also significantly positively correlated with the superoxide anion clearance rate,iron ion reduction ability and anti-asian oleic acid oxidation showed a very significant positive correlation(P<0.01).

     

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