Abstract: The establishment of respiration rate model of fruit is one of keys to design of MAP. In this paper,shiitake was used as the material,and the changes of respiration rate were measured under different temperature(T)and relative humidity(RH)conditions by the closed system method. Through single factor experiment and based on the principle of enzyme kinetics,the Arrhenius equation and the linear equation were used to obtain the parameters of the equation under different storage conditions. A two-factor crossover test was designed. Based on the Michaelies-Menten equation and the Arrhenius equation,the maximum respiration rate(Vm)model of shiitake based on the influence of T and RH was established by characterizing the relationship between relative humidity and the parameters of Arrhenius equation. The model's coefficient of determination exceeded 0.9. The Vm of shiitake could be obtained at the storage temperature from 5 to 25 ℃ and the RH from 33% to 95% based on the model.