Abstract: A method for determination of the content of six kinds of insecticide of phenyrazole derivatives including fipronil,fipronil-desulfinyl,fipronil-sulfone,fipronil-sulfide,ethiprole,flufiprole in poultry derived foods by QuEChERS method combined with liquid chromatography-mass spectrometry detection was established. The samples were extracted with acetonitrile,and purified with QuEChERS. The mass spectrometry analysis used ESI-negative-MRM method. The results showed that,the LOQs of six find of phenylpyrazole derivatives were different in samples of different matrixes,the LOQs range were 0.06~1.20 μg/kg.It was showed good linearities(R²>0.994)in different ranges of 1~40,4~160,10~400 μg/kg.Considering the difference of three kinds of sample,three levels of addition tests were carried out,the average recoveries were 84.49%~107.86%,the relative standard deviation(RSD)were 0.99%~4.19%. The method was rapid,sensitive and precise,and could be suitable for determination of insecticide of phenylpyrazole derivatives in poultry derived food.