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中国精品科技期刊2020
曹叶霞, 王泽慧, 贺金凤, 左鑫. 静乐黑枸杞多糖的提取及抗氧化性分析[J]. 食品工业科技, 2019, 40(14): 196-202. DOI: 10.13386/j.issn1002-0306.2019.14.032
引用本文: 曹叶霞, 王泽慧, 贺金凤, 左鑫. 静乐黑枸杞多糖的提取及抗氧化性分析[J]. 食品工业科技, 2019, 40(14): 196-202. DOI: 10.13386/j.issn1002-0306.2019.14.032
CAO Ye-xia, WANG Ze-hui, HE Jin-feng, ZUO Xin. Extracting Polysaccharide from Lycium ruthenicum of Jingle and Its Antioxidant Acitvity[J]. Science and Technology of Food Industry, 2019, 40(14): 196-202. DOI: 10.13386/j.issn1002-0306.2019.14.032
Citation: CAO Ye-xia, WANG Ze-hui, HE Jin-feng, ZUO Xin. Extracting Polysaccharide from Lycium ruthenicum of Jingle and Its Antioxidant Acitvity[J]. Science and Technology of Food Industry, 2019, 40(14): 196-202. DOI: 10.13386/j.issn1002-0306.2019.14.032

静乐黑枸杞多糖的提取及抗氧化性分析

Extracting Polysaccharide from Lycium ruthenicum of Jingle and Its Antioxidant Acitvity

  • 摘要: 为确定静乐黑枸杞多糖的最佳提取条件及其抗氧化性,以静乐黑枸杞为原料,水作提取剂,采用超声辅助提取静乐黑枸杞多糖。首先研究了液料比、超声功率、超声时间、超声温度四个因素对静乐黑枸杞多糖得率的影响,然后利用Box-Behnken设计原理,采用四因素三水平响应面分析法,得到以多糖得率为响应值的结果,同时探究该工艺条件下静乐果枸杞多糖的抗氧化性。结果得出最佳提取条件液料比、超声时间、超声功率、超声温度依次为40:1 (mL/ g)、50 min、360 W、70 ℃,在此条件下,多糖的得率为13.89%,与理论预测值的相对误差为0.22%,能够很好地与理论预测值吻合。静乐黑枸杞提取物对DPPH·、· OH和ABTS+·清除能力以及总还原能力与提取物的浓度呈量效关系,其中对DPPH·、· OH和ABTS+· IC50分别为0.125、6.00、0.40 mg·mL-1,结果为进一步静乐黑枸杞的合理开发利用提供了理论依据。

     

    Abstract: The optimum extraction condition of polysaccharides of Lycium ruthenicum of Jingle was determined and its antioxidant acitvity was studied. Polysaccharides from Jingle's Lycium ruthenicum were extracted with water solution by ultrasonic-assisted method. The effects of liquid-to-material ratio, ultrasonic power, ultrasonic time and ultrasonic temperature on the yield of polysaccharide from Jingle's Lycium ruthenicum were studied at first. Then the Box-Behnken design principle and four-factor three-level response surface methodology were adopted, the results were obtained with the yield of polysaccharides as the response value, and its antioxidant activities were studied. The result showed that the optimized conditions were ultrasonic power 360 W, liquid-to-material ratio 40:1 (mL/g), extraction temperature 70℃, extraction time 50 min. Under such conditions, the polysaccharide yield was 13.89%, and the relative error was 0.22%, which was well matched with the predicted yield. The scavenging activity of 2, 2-diphenyl-1-picry-hydrazyl radicals, hydroxyl radicals, ABTS+ radicals and reducing capacity showed that its extracts had dose-effect relationship with the increasing of concentration. The IC50 of DPPH·,·OH and ABTS+·were 0.125, 6.00 and 0.40 mg·mL-1, respectively. Therefore, it would provide a theoretical basis for further rational development and utilization of Jingle's Lycium ruthenicum.

     

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