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中国精品科技期刊2020
姚泽晨, 张根义. 植物乳杆菌微胶囊包载效果、物化性质及其胃肠道性能[J]. 食品工业科技, 2019, 40(14): 112-117,133. DOI: 10.13386/j.issn1002-0306.2019.14.019
引用本文: 姚泽晨, 张根义. 植物乳杆菌微胶囊包载效果、物化性质及其胃肠道性能[J]. 食品工业科技, 2019, 40(14): 112-117,133. DOI: 10.13386/j.issn1002-0306.2019.14.019
YAO Ze-chen, ZHANG Gen-yi. Encapsulation Effect, Physicochemical and Gastrointestinal Properties of Lactobacillus plantarum Microcapsules[J]. Science and Technology of Food Industry, 2019, 40(14): 112-117,133. DOI: 10.13386/j.issn1002-0306.2019.14.019
Citation: YAO Ze-chen, ZHANG Gen-yi. Encapsulation Effect, Physicochemical and Gastrointestinal Properties of Lactobacillus plantarum Microcapsules[J]. Science and Technology of Food Industry, 2019, 40(14): 112-117,133. DOI: 10.13386/j.issn1002-0306.2019.14.019

植物乳杆菌微胶囊包载效果、物化性质及其胃肠道性能

Encapsulation Effect, Physicochemical and Gastrointestinal Properties of Lactobacillus plantarum Microcapsules

  • 摘要: 以阿拉伯木聚糖与海藻酸钠为壁材,植物乳杆菌为芯材,通过锐孔凝固浴法制备植物乳杆菌微胶囊。本文拟从包载效果、物化性质及胃肠道性能方面对微胶囊进行研究。结果表明,微胶囊包埋产率73.60%±0.01%,包埋效率81.27%±0.17%,有效载量(3.68±0.4)×1011cfu/g。此外,微胶囊休止角(35.25±4.35) °、水分含量8.82%±0.01%、密度(0.25±0.01) g/cm3。SEM图像表明:微胶囊表面结构完整且致密,对植物乳杆菌有较好的保护效果。在胃肠道模拟实验中,微胶囊显示出较低的胃液溶解性和较高的肠液溶解性。耐酸性实验中,载菌微胶囊菌体数量仅下降1.2lg(cfu/mL),而菌悬液下降6.4lg(cfu/mL)。肠道释放实验中,载菌微胶囊1 h即可释放完全。在连续胃肠道实验中,微胶囊菌体数量仅下降0.55lg(cfu/mL),而菌悬液下降5.6lg(cfu/mL)。储藏性评估实验中,微胶囊在-20、4以及28 ℃条件下储藏7周,菌体数量分别下降1.2lg、1.7lg以及5.2lg(cfu/mL),表明低温储存对菌体保存更有利。综上所述,此微胶囊对植物乳杆菌有较好的包载效果。

     

    Abstract: The main purpose of this research was to study the effects of encapsulation, physicochemical properties and gastrointestinal performance of Lactobacillus plantarum microcapsules, which were prepared via the sharp-hole coagulation technique using arabinoxylan and sodium alginate as wall materials and Lactobacillus plantarum as a core material, respectively. The encapsulation yield was 73.60%±0.01%, the embedding efficiency was 81.27%±0.17% and the effective load was (3.68±0.4)×1011 cfu/g. Furthermore, The angle of repose was (35.25±4.35)°, the moisture content was 8.82%±0.01% and the density was (0.25±0.01) g/cm3. The SEM images clearly showed the complete and compact surface structure of the microcapsules, therefore, it had a good protective effect on Lactobacillus plantarum. The results obtained from gastrointestinal simulation indicated that the microcapsules had low acid solubility and high intestinal solubility. The acid resistance test showed a decrease in the number of bacteria loaded in microcapsules by only 1.2lg (cfu/mL) whereas bacterial suspension decreased by 6.4lg (cfu/mL). The intestinal release test showed that the microcapsules were completely released after 1 h. The number of bacteria loaded in microcapsules decreased by only 0.55lg (cfu/mL) whereas the bacterial suspension decreased by 5.6lg (cfu/mL) for the continuous gastrointestinal tests. Additionally, the microcapsules were stored at -20, 4 and 28℃ for 7 weeks, respectively. The number of bacteria decreased by 1.2lg, 1.7lg and 5.2lg (cfu/mL), which indicated that low temperature storage was more beneficial for the preservation of Lactobacillus plantarum. In summary, the microcapsule had a good encapsulation effect on Lactobacillus plantarum and could provide sufficient protection for it.

     

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