Abstract: Athyrium mengtzeense Hieron were extracted by ethanol, and then partitioned by system solvents with petroleum ether, ethyl acetate, butanol and water to afford four different fractions and their total phenolics content were analyzed by Folin-Ciocalteu method. In addition, radicals scavenging (DPPH and ABTS), α-glucosidase inhibitory, lipypolysaccharide-induced RAW264.7 cells assays were carried out to evaluate their antioxidant, hypoglycemic and anti-inflammatory activities. The results showed that the ethyl acetate fraction had the highest total polyphenols contents ((258.6±1.7) mg/g) and all the extracts from Athyrium mengtzeense possessed scavenging capacities for both DPPH and ABTS radicals in different degree, the ethyl acetate fraction was in the highest degree, this fraction also showed an equally α-glucosidase inhibitory activity, whose IC₅₀ was (93.4±3.7) μg/mL as that of positive control (acarbose).The petroleum ether fraction (12.5~50.0 μg/mL) displayed a significant inhibitory effect on NO production in LPS-induced RAW264.7 cells, but no obvious effect on cell proliferation.