• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
吴国萍, 周亚红, 李静泉, 李家伟. 表面增强拉曼光谱测定保健品中非法添加物西地那非[J]. 食品工业科技, 2019, 40(11): 254-259,264. DOI: 10.13386/j.issn1002-0306.2019.11.042
引用本文: 吴国萍, 周亚红, 李静泉, 李家伟. 表面增强拉曼光谱测定保健品中非法添加物西地那非[J]. 食品工业科技, 2019, 40(11): 254-259,264. DOI: 10.13386/j.issn1002-0306.2019.11.042
WU Guo-ping, ZHOU Ya-hong, LI Jing-quan, LI Jia-wei. Surface Enhanced Raman Spectroscopy(SERS) for the Detection of Sildenafil in Health Care Products[J]. Science and Technology of Food Industry, 2019, 40(11): 254-259,264. DOI: 10.13386/j.issn1002-0306.2019.11.042
Citation: WU Guo-ping, ZHOU Ya-hong, LI Jing-quan, LI Jia-wei. Surface Enhanced Raman Spectroscopy(SERS) for the Detection of Sildenafil in Health Care Products[J]. Science and Technology of Food Industry, 2019, 40(11): 254-259,264. DOI: 10.13386/j.issn1002-0306.2019.11.042

表面增强拉曼光谱测定保健品中非法添加物西地那非

Surface Enhanced Raman Spectroscopy(SERS) for the Detection of Sildenafil in Health Care Products

  • 摘要: 基于表面增强拉曼光谱技术(SERS),采用自制表面增强试剂(纳米金)与厂家提供的表面增强试剂,对比研究了对21种查缴的保健品样品中的非法添加物西地那非的快速拉曼检测效果,同时考察了不同配比纳米金和不同浓度的盐酸、硝酸和硫酸作为助剂的增强效果。结果表明,自制纳米金表面增强试剂(氯金酸和柠檬酸钠的体积比1:1)及2.67 mol·L-1的硝酸表面增强效果为佳,可对浓度为0.01 μg·mL-1西地那非阳性检出;与飞行时间-液质联用仪(QTOF-LC-MS)分析结果对比,自制纳米金检测吻合度为100%,厂家提供的增强试剂检测吻合度为86.4%。综上,自制表面增强试剂(纳米金)表面增强效果不差于厂家提供的表面增强试剂。

     

    Abstract: Based on surface-enhanced raman spectroscopy(SERS),the illegal additive sildenafil in 21 samples of seized health care products was detected by self-made nano-gold as the surface-enhanced reagents comparing with those provided by the manufacturers. The enhancement effects of nano-gold in various proportions with using diverse concentrations of hydrochloric acid,nitric acid and sulfuric acid were additionally measured. The results showed that:As the minimum detection limit of sildenafil was 0.01 μg·mL-1,the enhancement effect of self-made nano-gold as the surface-enhanced reagent(the volume of cholor-gold acid and sodium citrate was in the proportion 1:1)added nitric acid with the concentration of 2.67 mol·L-1 was as result the best among the all tests,so that it was 100% consistent with the results analyzed by QTOF-LC-MS,while those provided by the manufacturer were only 86.4% consistent. This indicated that the surface enhancement effect of the self-made surface enhancement reagent(nano-gold)was not inferior to that provided by the manufacturer.

     

/

返回文章
返回