Abstract: The aim of this study was to comprehensively evaluate the quality traits of the Euryale Ferox Salisb which was collected from 13 different cultivation areas or planting bases in Jiangsu,Anhui and Jiangxi province from the appearance,main nutrients,active ingredients and microelements by using High Performance Liquid Chromatography and Inductively Coupled Plasma Optical Emission Spectrometer,etc. Results indicated that there were variant differences in the appearance and content of the components of Euryale Ferox Salisb in different varieties and origins. South Euryale had large individuals and rounded kernels,and there was a significant difference in the fruit shape index,quality of the fruit,thickness of the fruit shell,and rate of seed kernels between South Euryale and North Euryale(p<0.05). The starch content of Euryale kernels in Yangzhou City of Jiangsu province and Shangrao City of Jiangxi Province was 76.84% and 76.53%,respectively,which was significantly higher than that of others(p<0.05). The highest crude protein content(13.61%)was in the Huaian city of Jiangsu province. While,the fat content(0.66%)was the highest in the Shangrao city of Jiangxi province,and was significantly higher than that in other producing areas(p<0.05). The contents of element K(1458.90 μg/g),Mg(348.95 μg/g),P(1590.67 μg/g)and Zn(38.39 μg/g)in the Tianchang city of Anhui province were higher than those of others,but the elements Ca(90.79 μg/g),Mn(15.99 μg/g)content was lower than other origin. The chromatographic fingerprints of Euryale from 13 production sites was established,and 12 common peaks were identified. The sample similarity was greater than 0.84. According to the principal component analysis,the 23 indexes reduced to 6 indicators,which reflected 91.20% of the original variables. In the cluster analysis,13 samples were clustered into 2 classes. The South Euryale in different regions were clustered into one class,and the rest of North Euryale were clustered into another one. The quality of Euryale Ferox Salisb were greatly affected by varieties. In summary,the multicomponents determined in this study provided a theoretical basis for the quality control and identification of tamping.