Abstract: The simultaneous determination method of six kinds of polyphenols content in kiwifruit peel was made by using the high performance liquid chromatography(HPLC). In terms of the specific approach,the InertSustain C18(4.6 mm×250 mm,5 μm)was applied to the chromatographic column. The gradient elution was carried out by using methanol-10 mmoL/L KH₂PO₄ aqueous solution(pH2.0)as mobile phase. The column temperature was 40 ℃,the flow rate was 1 mL/min,the sample volume was 10 μL,and the detection wavelength was detected at 280 nm. Results showed that this method could be used for separating the six target components well in 50 min. The approach had great reproducibility(RSD≤ 1.40%),strong stability(RSD≤ 2.86%)and high precision(RSD≤1.85%). Furthermore,the recovery rate was between 84.90% and 108.78%. Therefore,this was a simple and quick operation method which could effectively provide relevant data for the research of polyphones in kiwifruit peel.