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中国精品科技期刊2020
曾志鲁, 范慧群, 黄克愁, 罗舜菁, 叶江平, 刘成梅, 罗舜芬. β-环糊精结合脱支处理对重结晶大米淀粉消化性和结构的影响[J]. 食品工业科技, 2019, 40(10): 6-10,17. DOI: 10.13386/j.issn1002-0306.2019.10.002
引用本文: 曾志鲁, 范慧群, 黄克愁, 罗舜菁, 叶江平, 刘成梅, 罗舜芬. β-环糊精结合脱支处理对重结晶大米淀粉消化性和结构的影响[J]. 食品工业科技, 2019, 40(10): 6-10,17. DOI: 10.13386/j.issn1002-0306.2019.10.002
ZENG Zhi-lu, FAN Hui-qun, HUANG Ke-chou, LUO Shun-jing, YE Jiang-ping, LIU Cheng-mei, LUO Shun-fen. Effect of β-cyclodextrin Combined with Deranching on Digestibility and Structure of Recrystallized Rice Starch[J]. Science and Technology of Food Industry, 2019, 40(10): 6-10,17. DOI: 10.13386/j.issn1002-0306.2019.10.002
Citation: ZENG Zhi-lu, FAN Hui-qun, HUANG Ke-chou, LUO Shun-jing, YE Jiang-ping, LIU Cheng-mei, LUO Shun-fen. Effect of β-cyclodextrin Combined with Deranching on Digestibility and Structure of Recrystallized Rice Starch[J]. Science and Technology of Food Industry, 2019, 40(10): 6-10,17. DOI: 10.13386/j.issn1002-0306.2019.10.002

β-环糊精结合脱支处理对重结晶大米淀粉消化性和结构的影响

Effect of β-cyclodextrin Combined with Deranching on Digestibility and Structure of Recrystallized Rice Starch

  • 摘要: 为了提高抗性淀粉(RS)的含量,探究重结晶大米淀粉(RRS)、β-环糊精重结晶大米淀粉(β-CD-RRS)、重结晶脱支大米淀粉(DB-RRS)、β-环糊精重结晶脱支大米淀粉(DB-β-CD-RRS)的消化性和结构的差异。采用体外模拟消化法测定其消化性,通过X-射线衍射(XRD)、傅立叶变换红外光谱(FT-IR)、扫描电子显微镜(SEM)和差示扫描量热法(DSC)对其晶体结构、形貌和热稳定性进行表征。体外消化实验显示:RRS、β-CD-RRS、DB-RRS和DB-β-CD-RRS的抗性淀粉含量分别为16.45%、19.03%、43.83%和52.34%;蒸煮后,RRS、β-CD-RRS、DB-RRS和DB-β-CD-RRS的抗性淀粉含量分别为1.54%、5.73%、15.49%和20.18%。XRD分析表明,β-CD-RRS与RRS没有明显的衍射峰,DB-RRS和DB-β-CD-RRS均呈B+V型结晶,结晶度分别为23.6%和30.13%。但DB-β-CD-RRS的XRD图谱中在5.2 °处出现衍射峰,这表明形成了淀粉-β-环糊精复合物。SEM结果显示:RRS和β-CD-RRS呈片状结构,而DB-RRS和DB-β-CD-RRS呈紧密的球形颗粒结构。结果表明,β-环糊精结合脱支处理可促进淀粉重结晶及淀粉-β-环糊精复合物的形成,从而协同增加抗性淀粉的含量。

     

    Abstract: To improve the contents of resistant starch(RS),explore the recrystallized rice starch(RRS),the digestibility and structural difference of β-cyclodextrin recrystallized rice starch(β-CD-RRS),recrystallized detached rice starch(DB-RRS),β-cyclodextrin recrystallized de-branched rice starch(DB-β-CD-RRS)were investigated. The digestibility was evaluated by simulated digestion in vitro. The structure,thermal stability,and morphology of starches were characterized by X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FT-IR),and scanning electron microscopy(SEM),differential scanning calorimetry(DSC). Digestion expetiment in vitro showed that:The RS contents of RRS,β-CD-RRS,DB-RRS and DB-β-CD-RRS were 16.45%,19.03%,43.83% and 52.34%,respectively. After cooking,the RS contents of RRS,β-CD-RRS,DB-RRS and DB-β-CD-RRS were 1.54%,5.73%,15.49% and 20.18%,respectively. XRD analysis indicated that recrystallized rice starch(RRS)and recrystallized rice starch with β-CD(β-CD-RRS)were amorphous. However,recrystallized debranched rice starch(DB-RRS)and recrystallized debranched rice starch with β-CD(DB-β-CD-RRS)contained B+V type crystalline and their crystallinity was 23.6% and 30.13%,respectively. Specially,a new diffraction peak at 5.2 ° appeared in the XRD spectrum of DB-β-CD-RRS,which might result from the starch-β-CD complex. SEM analysis showed that RRS and β-CD-RRS were flaky,while DB-RRS and DB-β-CD-RRS were compact spherical particles. These results indicated that the combination of debranching and β-CD could promote the recrystallization of starch and the formation of starch-β-CD complex,thus synergistically increasing the RS contents.

     

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