核酸适配体在食源性致病菌检测中应用的研究进展

Research Progress on Utilization of Nucleic Acid Aptamer in Detection for Foodborne Pathogenic Bacteria

摘要: 核酸适配体(aptamer)是经体外筛选可特异性识别并结合靶分子的寡核苷酸片段(单链DNA/RNA序列),具有高特异性、高灵敏度、高亲和力及易于修饰等优点,已在食源性致病菌检测领域得到应用。本文主要对核酸适配体筛选技术分类以及纳米金适配体技术、荧光适配体技术、电化学适配体技术、流式细胞适配体技术和表面增强拉曼适配体技术在食源性致病菌检测中的应用作简要综述。

Abstract: The aptamer is an oligonucleotide fragment(single-stranded DNA/RNA sequence)which can specifically recognize and bind to target molecules by screening in vitro. It has the obvious advantages of high specificity and sensitivity,high affinity and simplicity of modification,and has been applied in the field of foodborne pathogenic bacteria detection. The classification of aptamer screening technologies and nanogold aptamer technology,fluorescence aptamer technology,electrochemical aptamer technology,flow cytometry technology and surface enhanced Raman aptamer technology in the detection of foodborne pathogenic bacteria were mainly reviewed.