Abstract: The preventive effects of polyphenols of large-leaved Kuding tea(PLK)on CCl₄-induced hepatic damage in mice were determined in this study. The Kunming male mice were given low(50 mg/kg)and high(100 mg/kg)concentration of PLK,and drug of silymarin(100 mg/kg)by gavage for 14 d,then the animal model of acute liver injury was established by intraperitoneal injection of 0.8% CCl₄.The blood was collected from the ophthalmic venous plexus of mice. Liver weight,liver index,serum markers and expression of oxidative and inflammatory related mRNA genes in liver tissues were measured after the mice were executed. The experiment results showed that PLK could reduce the liver index in hepatic damage mice,meanwhile,and PLK also could reduce the serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),triglyceride(TG),total cholesterol(TC)levels. Pathological observation also showed that PLK could effectively reduce the hepatictissue destruction and hepatocellular necrosis caused by CCl₄.The qPCR experiment results showed that PLK could significantly increase copper/zinc-superoxide dismutase(Cu/Zn-SOD),manganese-superoxide dismutase(Mn-SOD),nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-alpha(IκB-α),catalase(CAT)mRNA expressions and decrease cyclooxygenase-2(COX-2),inducible nitric oxide synthase(iNOS),tumor necrosis factor-alpha(TNF-α),nuclear factor-kappa B(NF-κB),interleukin 1 beta(IL-1β)expressions of hepatic tissues in mice compared with the mice in control group(p<0.05). PLK had good preventive effect on CCl₄ induced hepatic damage,and PLK had the similar effects with silymarin,which has good functional effect.