Abstract: Fresh-cut apples were used as the experimental material,and were coated with the chitosan oligosaccharide solutions of different concentrations(1,1.5 and 2 g/100 mL),and stored at 4 ℃. The total number of colonies,mold,yeast and coliforms were measured,and their respiratory intensity,soluble solids,titratable acid,ascorbic acid(Vitamin C,V_C)contents,browning index,hardness,weight loss rate,and hysiological indicators polyphenols oxidase(PPO)and peroxidase(POD)activity were measured. The results showed that the chitosan oligosaccharide coating could inhibit the microbial growth of fresh-cut apples obviously,at the same time,it could effectively inhibit the increasing of the respiration intensity,delay the loss of it’s soluble solid and titratable acid,and decrease the weightlessness rate and prevent the softening. However,chitosan oligosaccharide coating treatment would increase activities of PPO and POD,and increase the browning index(BI)of fresh-cut apples. In comparison,1.5 g/100 mL chitosan oligosaccharide coating treatment had the best effect on inhibiting the growth and retarding nutrient loss of fresh-cut apple.