Abstract: The distribution of yeast species are closely related to geographical location and climatic conditions. In our country (China), most of wine producing regions are located in north latitude 35 to 40°N, only a little region located in north latitude 28 to 30°N in Yunnan province. This region has complex topography and diversity climate types, which provide superior conditions for the research and development of high quality wine yeast. In this review, the research progress of yeast biodiversity in Yunnan wine-producing regions and the application of these characteristic yeast strains in wine making application were described. It was expected to provide references for the development of local wine yeast resources and the production of characteristic wine.