枣阳酸浆水中乳酸菌的可培养多样性及其分离方式评价

张振东; 舒娜; 倪慧; 尚雪娇; 马佳佳; 赵慧君; 马磊; 郭壮

doi:10.13386/j.issn1002-0306.2019.04.021

枣阳酸浆水中乳酸菌的可培养多样性及其分离方式评价

Diversity of Lactic Acid Bacteria from Suanjiangshui in Zaoyang and Its Evaluation of Isolation Method

摘要

摘要: 采用变形梯度凝胶电泳(DGGE)对浆水样品细菌群落进行分析,使用6种常用的乳酸菌分离方式对酸浆水中的乳酸菌进行了分离与鉴定,并对这些方法的分离效果进行了评价。结果表明,酸浆水样品中乳酸菌的数量约为5×10⁶~2.5×10⁸ cfu/mL,不同来源的浆水样品中乳酸菌数量差异较大。通过这6种培养方式共分离得到90株乳酸菌。经过鉴定,它们是德氏乳杆菌(Lactobacillus delbrueckii)、发酵乳杆菌(Lactobacillus fermentium)、副干酪乳杆菌(Lactobacillus paracasei)、植物乳杆菌(Lactobacillus plantarum)、鼠李糖乳杆菌(Lactobacillus rhamnosus)与玉米乳杆菌(Lactobacillus zeae)。在6种乳酸菌分离方式中,非增菌法中厌氧罐-厌氧袋法、厌氧工作站(10% H₂,10% CO₂,80% N₂)及增菌法对酸浆水中的乳酸菌分离效果较好。

Abstract: DGGE was used to analyze the bacterial community of Suanjiangshui samples, and the microbial compositions in Suanjiangshui were separated and identified by six common lactic acid bacteria separation methods, and the separation effects of these methods were evaluated. The results showed that there were differences in number of lactic acid bacteria among the Suanjiangshui from different sources. The number of lactic acid bacteria were about 5×10⁶~2.5×10⁸ cfu/mL. A total of 90 strains of lactic acid bacteria were obtained, which were identified as Lactobacillus delbrueckii, Lactobacillus fermentium, Lactobacillus zeae, Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus paracasei. Among the methods used in this study, anaerobic jar with anaeropack, anaerobic workstation (10% H₂, 10% CO₂, 80% N₂) and enumeration by litmus milk medium method were the best methods for LAB isolation.

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