Abstract: In order to obtain strains with excellent production performance, the PDA solid medium plate coating method of sampling was to quantitatively separate and purify yeast from salted fish, and then studied the growth temperature, the acid resistance and nitrite tolerance ability of dominance salt-tolerant yeast strains. The results showed that, 436 strains of yeast were isolated from 15 kinds of salted marine fish and 9 strains of yeast were obtained after purification. Finally, 7^# Meyerozyma guilliermondii and 9^# Kodamaaea ohmen with the better salt tolerance ability were selected by cultivated with YPD liquid medium of different NaCl contents from 9 strains of yeast, and salt tolerances reached 9% and 12% respectively. And they were found that the optimum growth temperature were between 28~32℃, the optimum pH was between 5~6, the optimum contents of nitrite was less than 100 mg/kg. The dominant strains obtained from the salted fish had good growth performance and could be the basis for the development of the starter of the salted fish products.