Abstract: This study was carried out to optimize the acidic extraction of total alkaloids from Maca with different color type. The effects of three extraction parameters including solid-to-liquid ratio, temperature and duration on extraction efficiency were investigated by orthogonal array design. Results were as follows:The order of effect of different factors on extraction efficiency for yellow Maca and black Maca were solid-to-liquid ratio > duration > temperature. The order of effect of different factors on extraction efficiency for purple Maca were duration > solid-to-liquid ratio > temperature. The content of total alkaliods in yellow Maca was 9.89%±0.38% under the optimum extracting conditions:Ratio of solid to liquid 1:30, temperature 75℃, time 5.5 h.The optimal extraction condition of purple Maca were 90℃ temperature for 5.5 h with solid-to-liquid ratio 1:25, and the yield of total alkaloids was 10.36%±0.58%. The optimal extraction parameters for black Maca was as follows:Temperature 85℃, time 6 h, solid-liquid ratio 1:20, under these conditions, the extraction yield of total alkaloids was 10.01%±0.78%. In conclusion, the optimal extraction conditions for total alkaloids in different color types Maca had some differences.