Abstract: Gossypol is a toxic chemical compound which may exist in agricultural products like cottonseed, cottonseed oil, cottonseed meal and so on, the addition of virus-free cottonseed meal which is incomplete as a protein source in animal feed may cause gossypol accumulation in animals and cause potential harm to human health through food chain. Therefore, gossypol, including eggs and milk, may also contain gossypol. Combined with gossypol toxicity, the method of detection of gossypol content in agricultural products was summarized in this paper, and the molecular imprinting technique was advised after analyzing and concluding, so as to provide references for establishing a more efficient, green, fast and accurate detection method.