Abstract: Bacterioncin is one of the inhibitory metabolites produced by certain bacteria. It has advantages of efficiency,non-toxic as well as no drug resistance,as a result,bacteriocins have been concerned as the biopreservative agent in recent years. Currently,only a limited number of bacteriocins are used in the actual production because of the limitations of their own nature,and they cannot play due effect in any environments. Therefore,only by constanty digging fresh and efficient bacteriocin products can we promote biological preservation technology development. At present,the main detection methods of antibacterial activity of bacteriocins are agar diffusion method and turbidimetric method. With the advancement of technology,some new and efficient methods such as radiation method,ELISA kit and bioengineering technology are expected to become effective alternative methods. This review focused on some problems that should be paid attention to in the preparation of the experiment. In addition,we compared the method of determination of antibacterial activity and the expression of bacteriocin titer in order to improve the normative expression of the results and provide the theoretical basis.