果胶酶处理对甜玉米芯可溶性糖含量的影响

Effect of Pectinase Treatment on the Content of Soluble Sugar of Sweet Corncob

摘要: 为提高甜玉米芯总糖产量,对甜玉米芯进行果胶酶水解。采用单因素实验考察酶添加量、pH、温度以及时间对果胶水解度的影响,采用响应曲面法分析建立二次回归模型。结果表明,果胶水解的最佳条件为:酶添加量88 U/g,温度60 ℃,pH5.0,时间90 min,此时果胶水解度为43.82%±0.02%。分别取果胶酶处理前后甜玉米芯进行可溶性糖含量对比分析,果胶酶处理前可溶性糖含量为53.16%±0.02%,果胶酶处理后为62.89%±0.03%,可溶性糖含量提高了9.73%。

Abstract: To increase the total sugar yield of sweet corncobs,the pectinase hydrolysis of sweet corncob was performed. Four factors of reactions were researched including enzyme addition,pH,temperature and time through single factor experiment. And the optimum extraction conditions of pectin hydrolysis were obtained by and response-assisted surface methods. The results showed that the optimum conditions for the hydrolysis of pectin as follows:Enzyme addition amount,temperature,pH and time was 88 U/g,60 ℃,5.0 and 90 min,respectively,at which the pectinase hydrolysis could reach 43.82%±0.02%. The soluble sugar content of sweet corn cobs before and after pectinase treatment was compared. The soluble sugar content before pectinase treatment was 53.16%±0.02%,the pectinase treatment was 62.89%±0.03%,and the soluble sugar content was increased by 9.73%.