Abstract: The effect of 2,4-epibrassinolide(EBR)treatment on the quality and physiological characteristics of sweet cherry(cv. Van)during storage were studied. Results showed that 5 μmol/L EBR treatment could effectively reduce the incidence of browning and decay of pulp and peduncle during storage,delay the color change of peel and pulp and increase malondialdehyde content,delay the firmness and soluble solids content,and inhibit the activity of polyphenoxidase,peroxidase and lipoxygenase,increased the activity of catalase and superoxide dismutase. EBR treatment could maintain the quality of the fruit and flavor and delay the aging of sweet cherry than that of control.