Abstract: In this paper, by using the byproducts of wheat processing after decreasing viscosity and liquefaction, the wheat starch from byproducts was prepared as a glucose syrup. The addition of glucoamylase, the amount of pullulanase, the saccharification temperature and the saccharification pH's effect on the DE value were studied. The optimum technique parameters were obtained by using responses surface tests:the amount of glucose amylase was 300 U/g, the amount of pullulanase was 0.17 U/g, the saccharification temperature was 62℃, the saccharification pH was 3.9, the saccharification time was 36 h, and the DE value was 69.45%, and the effluent conversion rate was 78.70% and the total recovery of glucose was 23.82% by high performance liquid-evaporative light scattering test.This approves that it was practicable to produce glucose syrup from the byproduct of wheat processing, and it had important guiding significance for ptactical production.