Abstract: Objective:The main components of lavender essential oil(LO)of molecular distillation purification by headspace solid phase microextraction and gas chromatography(HS-SPME-GC)was studied. Methods:Based on single factor,the total peak areas of chromatogram extraction were the indexes by central composite design response surface method to analyze 4 factors and 3 levels of response surface analysis of the extraction temperature,equilibrium time,extraction time and analysis time of the headspace solid phase micro extraction. By the orthogonal experiment,the purity of main components of lavender essential oil was indicator to analyze molecular distillation of distillation temperature,distillation pressure and film speed by 3 factors and 3 levels analysis. Results:The optimum extraction conditions of headspace solid phase micro extraction were extraction temperature 70℃,extraction time 41 min,equilibrium time 10 min,analysis time 6 min and the average of peak area was 7750 in the optimized condition. The optimized conditions of molecular distillation were the distillation temperature of 55℃,distillation pressure of 0.6 kPa,film speed of 320 r·min^-1 and the purities oflinalyl acetate,linalool,Lavandayl acetate in lavender essential oil were 45.11%,25.52%,14.27% in the optimized condition. Conclusion:HS-SPME and molecular distillation technology were applied to the enrichment and purification of volatile components of lavender essential oil.