Abstract: The improvement effects of Kuding tea flavonoids extracts ( KFE) on D-galactose induced aging in mice were studied in this study. Aging moded mice induced by the D-galactose injection ( 120 mg/kg) were experimental subjects whose body weight, organ index, serum and tissue index were evaluating indicators. Experiment kit and RT-PCR detection methods were used for determination the effects of KFE ( 50 and 100 mg/kg) on serum and tissue of aging mice, the anti-aging effects of KFE were comprehensive investigated.The experiment results showed that KFE could significantly ( p < 0.05) improve the weight loss, thymus and brain organ index decreasing of mice by D-galactose treatment. KFE could raise total antioxidative capability ( T-AOC) level and reduce malondialdehyde ( MDA) and nitric oxide ( NO) levels, meanwhile KFE also increased enzyme activity of superoxide dismutase ( SOD) and glutathione peroxidase ( GSH-Px) in serum and hepatic tissue of aging mice. KFEalso increased significantly ( p < 0.05) raise neuronal nitric oxide synthase ( n NOS) , endothelial nitric oxide synthase ( e NOS) , manganese superoxide dismutase ( Mn-SOD) , copper zinc superoxide dismutase ( Gu/Zn-SOD) , catalase ( CAT) mRNA expressions and reduce inducible nitric oxide synthase ( i NOS) expression, and high concentration of KFE ( 100 mg/kg) showed the better effects than the same concentration of vitamin C.