一种薤白中性多糖的结构鉴定及体外抗氧化活性研究

Structural characterization and in vitro antioxidant activities of a neutral polysaccharide from Allium macrostemon Bunge

摘要: 采用分步醇沉和柱层析的方法,从薤白中分离纯化得到一种中性多糖AMP60N,其重均分子量为11200 u,由阿拉伯糖、葡萄糖和半乳糖构成,经红外光谱分析,同时结合高碘酸氧化、Smith降解及GC分析等手段,通过甲基化以及核磁共振波谱分析,初步确定了AMP60N主要由Glc(1→2)和Ara(1→5)构成,以Gal(1→6)和Glc(1→6)为支链的分子结构组成。对AMP60N清除各种自由基的研究表明其具有一定的体外抗氧化活性,呈现明显的量效关系,其抗氧化能力弱于V_C。

Abstract: By using the method of stepwise ethanol precipitation and column chromatography, a neutral polysaccharide named AMP60 N was isolated from Allium macrostemon Bunge.The molecular weight of AMP60 N was estimated to be 11200 u, which was composed of arabinose, glucose and galactose. AMP60 N was characterized by using the periodic acid oxidation, Smith degradation, methylation, FT-IR, GC, GC-MS, NMR, etc.The in vitro antioxidant assay of AMP60 N showed that it had certain antioxidant activities in vitro, which showed dose-effect relationship, oxidation vesistance was weaker than V_C.