头孢克肟诱导肠道菌群紊乱动物模型的建立
Establishment of mice model for intestinal dysbacteria induced by cefixime dispersible tablets
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摘要: 目的:构建头孢克肟诱导的小鼠肠道菌群紊乱模型。方法:将40只体重1822 g的SPF级BALB/c雌性小鼠随机分为正常对照组、头孢克肟低剂量组、中剂量组、高剂量组,每组10只。灌胃剂量分别为187.75 mg/（kg·d）,375.50 mg/（kg·d）,563.25 mg/（kg·d）,正常对照组灌胃相同剂量的蒸馏水。连续灌胃7 d后颈椎脱臼处死并收集盲肠内容物,采用传统培养结合聚合酶链式反应-变性梯度凝胶电泳技术（polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE）观察和分析头孢克肟对小鼠肠道菌群的影响。结果:高剂量组小鼠粪便中乳酸杆菌数量在所选稀释度下降至30CFU以下,而肠球菌数量上升至1.26×105CFU,大肠杆菌数量上升至1.86×106CFU。与空白对照组相比,模型组小鼠肠道菌群总体多样性和细菌种类显著降低。结论:中剂量和高剂量头孢克肟均能不同程度的诱导小鼠肠道菌群紊乱模型,并且肠道菌群紊乱程度随灌胃剂量的增加而加重,模型成功建立。Abstract: Objective: To produce the mouse models of intestinal dysbacteria which induced by cefixime dispersible tablets.Methods: female SPF grade BALB/c 36, weight 18~22 g, intestinal dysbiosis mice treated with cefixime dispersible tablets, low dose ( 187.75 mg/ ( kg· d) ) , medium dose ( 375.5 mg/ ( kg·d) ) and high dose ( 563.25 mg/ ( kg·d) ) . Control mice received sterile deionized water by oral gavage. On the 8th day, the mice were euthanized by cervical dislocation. The caecum contents were collected in a sterile 2 m L centrifuge tube. Compare the shift in the intestinal microbial by vital cell counting and polymerase chain reaction-denaturing gradient gel electrophoresis ( PCR-DGGE) analysis. Result: The Enterococcus and E. coli were promoted and Lactobacillus was suppressed. The reduction of diversity and abundance was shown in middle and higher doses group.Conclusion: The model of intestinal dysbacteria builded successfully.
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